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目的探讨小鼠肝癌细胞死亡方式对巨噬细胞活性的影响。方法制备凋亡、坏死的小鼠肝癌细胞,分别注入实验小鼠体内,收集小鼠腹腔巨噬细胞,检测各组小鼠巨噬细胞NO水平及细胞毒活性。结果注射凋亡细胞组小鼠巨噬细胞NO的分泌及细胞毒作用下降,注射坏死细胞组小鼠巨噬细胞NO的分泌及细胞毒作用升高,与对照组比较,差异均有显著性(P<0.05)。结论凋亡的小鼠肝癌细胞抑制巨噬细胞的细胞毒活性及NO的分泌,而坏死的小鼠肝癌细胞则使巨噬细胞的细胞毒作用及NO的分泌明显增强。
Objective To investigate the effect of mouse hepatocarcinoma cell death on macrophage activity. Methods Apoptotic and necrotic mouse hepatocarcinoma cells were prepared and injected into the experimental mice respectively. The peritoneal macrophages of mice were collected and the level of NO and the cytotoxic activity of the macrophages were detected. Results NO secretion and cytotoxicity of macrophages in the apoptotic cells were decreased and NO secretion and cytotoxicity of the macrophages in the injected necrotic cells group were increased. Compared with the control group, the differences were significant ( P <0.05). Conclusion The apoptotic mouse hepatoma cells inhibit the cytotoxic activity of macrophages and the secretion of nitric oxide, whereas the necrotic mouse hepatoma cells enhance the cytotoxicity and NO secretion of macrophages.