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运用Ca~(2+)指示剂Fura-2作为细胞内钙离子的荧光探针,利用AR—CM—MIC阳离子测定系统,检测了分离的神经细胞内游离钙及其变化,并观测了DGAVP和Org2766对蛋白质合成抑制剂茴香霉素(ANI)引起细胞内钙离子浓度([Ca~(2+)]_i)变化的影响。结果表明茴香霉素可使[Ca~(2+)]_i显著升高,且有量效关系;DGAVP本身并不引起[Ca~(2+)]_i发生显著变化,但适当剂量的DGAVP可显著对抗一定剂量范围内ANI升高[Ca~(2+)]_i的作用,提示DGAVP对抗ANI的蛋白质合成抑制效应可能是通过拮抗ANI升高[Ca~(2+)]_i这一途径实现的,另一神经肽Org2766则可能不是通过这一机制发生作用。从细胞内Ca~(2+)的角度看,这两种肽的作用机理显然是不同的。
Using Ca 2+ indicator Fura-2 as fluorescent probe of intracellular calcium ion, free calcium in isolated neurons was detected by AR-CM-MIC cation assay system and DGAVP and Effect of Org2766 on Changes of Intracellular Calcium Concentration ([Ca2 +] i) Induced by Anisomycin (ANI), a Protein Synthesis Inhibitor. The results showed that anisomycin induced a significant increase of [Ca ~ (2 +)] _ i and there was a dose-response relationship; DGAVP itself did not cause significant changes in [Ca ~ (2 +)] _i, Significantly antagonized the increase of [Ca2 +] i in ANI, suggesting that the inhibitory effect of DGAVP against ANI on protein synthesis may be through antagonizing the increase of [Ca2 +] i by ANI Of the other neuropeptide Org2766 may not play a role through this mechanism. From the perspective of intracellular Ca ~ (2+), the mechanism of action of these two peptides is apparently different.