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目的:观察川芎嗪后处理对大鼠心肌缺血再灌注损伤的保护作用及探讨其可能的作用机制。方法:采用结扎大鼠左冠状动脉前降支方法制备心肌缺血再灌注损伤模型,记录各组心律失常发生情况,测定肌酸激酶(CK)、超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)、一氧化氮合酶(NOS)含量,HE染色光镜下观察心肌组织形态学改变并秤重检测心肌梗死面积。结果:川芎嗪组的心律失常发生率明显降低,心肌细胞肿胀明显减轻,血中SOD、NO、NOS含量增加,MDA,CK的生成减少。(P<0.05)。结论:川芎嗪后处理能降低心律失常发生、减少坏死面积,其作用可能与提高SOD、NO、NOS含量,减少MDA生成有关。
Objective: To observe the protective effect of ligustrazine postconditioning on myocardial ischemia-reperfusion injury in rats and to explore its possible mechanism. METHODS: Myocardial ischemia-reperfusion injury model was established by ligation of the left anterior descending coronary artery in rats. The occurrence of arrhythmia in each group was recorded. Creatine kinase (CK), superoxide dismutase (SOD) and malondialdehyde were measured. (MDA), nitric oxide (NO), nitric oxide synthase (NOS) content, HE staining staining was used to observe the morphological changes of myocardium and to measure the infarct size of myocardium. RESULTS: The incidence of arrhythmia in the tetramethylpyrazine group was significantly reduced, myocardial cell swelling was significantly reduced, blood SOD, NO, NOS levels were increased, and MDA and CK production were decreased. (P<0.05). Conclusion: Tetramethylpyrazine post-treatment can reduce the occurrence of arrhythmia and reduce the area of necrosis. Its effect may be related to the increase of SOD, NO, NOS content and decrease of MDA production.