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为获得对黄瓜立枯病具有显著防效的优良菌株,以从果蔬体内、根际土壤等多生境中分离的100株细菌为出发菌株,采用平板对峙法和拌土法对100株供试菌株进行初筛和复筛。初筛得到8株能显著抑制立枯丝核菌(Rhizoctonia solani)的菌株(抑菌带直径D>0.60cm);复筛进一步分析表明,其中3株菌能够显著促进黄瓜幼苗的生长和高效防治苗期立枯病害,与对照相比,黄瓜幼苗壮苗指数分别增加16.22%(60)、13.48%(K13)和43.04%(Z84),相对防效分别达67.53%(60)、59.42%(K13)和77.27%(Z84);通过形态观察、生理生化试验及16SrDNA同源性序列对比分析,将60、K13和Z84菌株鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),可为蔬菜苗期高效微生物制剂的开发提供微生物资源。
In order to obtain an excellent strain with significant control effect on the blight of cucumber, 100 strains of bacteria isolated from multi-habitats such as fruits and vegetables and rhizosphere soils were used as starting strains. 100 strains of tested strains The initial screening and re-screening. Eight strains of Rhizoctonia solani were significantly screened (diameter of bacteriostasis zone D> 0.60cm) by preliminary screening. Further analysis showed that three strains of rhizoctonia solani could significantly promote cucumber seedling growth and high efficiency Compared with the control, the strong seedling index of Cucumber seedlings increased by 16.22% (60), 13.48% (K13) and 43.04% (Z84) respectively, and the relative control efficiency reached 67.53% (60) and 59.42% K13) and 77.27% (Z84) respectively. Through the morphological observation, physiological and biochemical tests and comparative analysis of 16S rDNA homology sequences, the strains 60, K13 and Z84 were identified as Bacillus amyloliquefaciens, The development of formulations provides microbial resources.