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[目的]拟建立液相色谱串联质谱法测定质量分数为4%的春雷霉素可湿性粉剂中春雷霉素的分析方法。[方法]根据农药性质,优化仪器条件,以ACQUITY UPLC BEH AMIDE不锈钢柱为色谱柱;0.2%甲酸水和乙腈为流动相;流速:0.25 mL/min;应用UPLC-MS/MS选择多反应监测(MRM)正离子模式扫描测定。[结果]在0.002~0.1 mg/L范围内,春雷霉素线性关系良好(r2=0.9997),回收率为97.2%~104.3%,相对标准偏差小于3.0%。[结论]方法精密度和准确度较高,是一种较为理想的检测分析方法。
[Objective] The research aimed to establish a method for the determination of kasugamycin in the kasugamycin wettable powder with the mass fraction of 4% by liquid chromatography-tandem mass spectrometry. [Method] According to the properties of pesticides and the optimized instrument conditions, ACQUITY UPLC BEH AMIDE stainless steel column was used as the column. 0.2% formic acid and acetonitrile were used as the mobile phase. The flow rate was 0.25 mL / min. MRM) positive ion mode scanning assay. [Result] The calibration curve of kasugamycin was good (r2 = 0.9997) in the range of 0.002-0.1 mg / L with the recovery of 97.2% -104.3% and the relative standard deviation less than 3.0%. [Conclusion] The method has high precision and accuracy and is an ideal detection and analysis method.