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目的研究肝细胞肝癌（ＨＣＣ）中细胞凋亡和血管形成的相互关系．方法采用原位末端标记技术和抗第Ⅷ因子的抗体分别检测ＨＣＣ凋亡指数和瘤内微血管的密度，同时用免疫组化法检测血管内皮细胞生长因子（ＶＥＧＦ）及其受体ｆｌｔ１和肝（癌）细胞凋亡启动基因ｆａｓ的表达．结果ＶＥＧＦ的表达主要见于微血管形成处的血管内皮细胞及其周围的瘤细胞和胆管上皮细胞，阳性率为８２７％，在ＨＣＣ组织中的表达具有普遍性．平均微血管计数为２８～４１６个／２００倍视野．在瘤组织中，微血管的密度越高的区域，ＶＥＧＦ的表达就越丰富．ＶＥＧＦ的受体ｆｌｔ１的表达见于血管内皮细胞和部分窦内皮细胞，特别是新生的微血管内皮细胞．ＶＥＧＦ阳性区域很少或不表达Ｆａｓ抗原．凋亡细胞的分布和凋亡指数与肿瘤的微血管密度呈明显的负相关（ｒ＝－０９１７，Ｐ＜００１）．结论ＨＣＣ中血管形成主要是由ＶＥＧＦ／ｆｌｔ１系统介导的．血管形成丰富的区域，细胞凋亡的敏感性和发生率降低
4. Objective To study the relationship between apoptosis and angiogenesis in hepatocellular carcinoma (HCC). Methods The in situ end labeling technique and anti-factor VIII antibody were used to detect the apoptosis index of HCC and the density of intratumoral microvessels. Meanwhile, immunohistochemical method was used to detect vascular endothelial growth factor (VEGF) and its receptor flt-1. Liver (cancer) cell apoptosis initiation gene fas expression. Results The expression of VEGF was mainly found in the vascular endothelial cells and its surrounding tumor cells and bile duct epithelial cells. The positive rate was 82.7%. The expression of VEGF was universal in HCC tissues. The average microvessel count was 2 8 41 6/200 times visual field. In the tumor tissue, the higher the density of microvessels, the richer the expression of VEGF. The expression of VEGF receptor flt 1 is found in vascular endothelial cells and some sinusoidal endothelial cells, especially neonatal microvascular endothelial cells. VEGF positive areas show little or no Fas antigen. The distribution of apoptotic cells and the apoptotic index were significantly negatively correlated with the microvessel density of the tumor (r=-0.917, P<0. 01). Conclusion The angiogenesis in HCC is mainly mediated by VEGF/flt1 system. In areas with rich blood vessels, the sensitivity and incidence of apoptosis decrease