目的和方法：本实验用９例胃癌患者ＴＩＬ和ＩＬ２在体外共同孵育后，用流式细胞仪分析胃癌ＴＩＬ的细胞表型特征。结果：ＴＩＬ细胞表型特征是以ＣＤ３为主，ＣＤ４／ＣＤ８为０８３，ＮＫ细胞１６３±３６％。经白细胞介素２（ＩＬ２）激活２０天后，ＣＤ３减少，ＣＤ４／ＣＤ８为１８５，ＮＫ细胞数量增至４１３±１３７％，Ｐ＜００１。ＬＤＨ释放法测定激活后的ＴＩＬ对自体胃癌细胞杀伤率比培养初期高３７４倍，变比对同时培养的７９０１胃癌细胞杀伤率高１７５倍。对自体和异体胃癌细胞杀伤作用均显著高于ＬＡＫ细胞，且具有靶细胞特异性。结论：结果表明胃癌ＴＩＬ对胃癌细胞杀伤作用，可能与ＮＫ细胞数量增多，导致胃癌细胞凋亡有关 Purpose and Methods: In this experiment, TIL and IL 2 in 9 patients with gastric cancer were incubated together in vitro, and the cell phenotypes of gastric TIL were analyzed by flow cytometry. RESULTS: The phenotypes of TIL cells were predominantly CD3, CD83/CD8 was 0.83, and NK cells were 16.3 ± 3.6%. After activation of interleukin-2 (IL-2) for 20 days, CD3 decreased, CD4/CD8 was 185, and the number of NK cells increased to 413±137%, P<001. LDH release assay showed that the killing rate of activated TIL on autologous gastric cancer cells was 374 times higher than that of the initial culture, and the killing rate of the 7901 gastric cancer cells cultured simultaneously was 175 times higher. The killing effect of autologous and allogeneic gastric cancer cells was significantly higher than that of LAK cells, and had target cell specificity. Conclusion: The results show that the cytotoxicity of gastric cancer TIL on gastric cancer cells may be related to the increase of NK cells and apoptosis of gastric cancer cells.