论文部分内容阅读
目的 观察大鼠延髓背角 (MDH)内向丘脑或臂旁核投射的Fos阳性神经元与γ 氨基丁酸 (GABA)或甘氨酸 (Gly)阳性终末的联系。 方法 四甲基罗达明 (TMR)逆行追踪结合TMR、Fos、GABA或Gly的免疫荧光三重染色技术。 结果 GABA或Gly阳性终末主要分布于延髓背角浅层 (Ⅰ、Ⅱ层 ) ;给予面口部痛刺激后 ,Fos阳性神经元也主要分布于浅层 ;TMR注入一侧丘脑或臂旁核 ,逆标神经元分别主要见于对侧或同侧MDH的浅层 ;部分逆标神经元呈Fos阳性 ;GABA或Gly阳性终末与Fos阳性投射神经元形成密切接触。 结论 MDH内感受面口部伤害性刺激信息的部分神经元向丘脑或臂旁核投射 ,GABA或Gly可能对这些伤害性感受神经元发挥抑制作用。
Objective To observe the association of Fos-positive neurons projecting to the medial thalamus or parabrachial nucleus of medullary dorsal horn (MDH) with γ-aminobutyric acid (GABA) or glycine (Gly) -positive terminals. Methods Tetramethylrhodamine (TMR) retrograde tracing was performed by immunofluorescence triple staining with TMR, Fos, GABA or Gly. Results The positive terminal of GABA or Gly was mainly located in the superficial layer of medullary dorsal horn (Ⅰ, Ⅱ). Fos positive neurons were also mainly distributed in the superficial layer after stimulation of facial pain. TMR was injected into the thalamus or parabrachial nucleus , The reverse-labeled neurons were mainly seen in the superficial layers of the contralateral or ipsilateral MDH; some of the anti-retroviral neurons were Fos-positive; GABA-or Gly-positive terminals were in close contact with Fos-positive neurons. Conclusion Some neurons in nodal stimulation of facial nerve in MDH project to the thalamus or parabrachial nucleus. GABA or Gly may inhibit these nociceptive neurons.