高产水稻品种 Pusa-33对稻长蠕孢菌(Helmintborium oryzae)引起的稻胡麻斑病敏感,因此在谷粒产量和质量上损失很大。本实验试图通过甲基磺酸乙酯(EMS)处理来诱发抗性突变。M_1代:水稻品种 Pusa-33的种子在蒸馏水中预浸12小时后,在24±1℃下,用0.15,0.30,0.45,0.60％EMS 的 pH5.0,7.0和9.0溶液处理6小时。每一处理及对照各100粒种子播在大田中。成熟时,M_1植株和对照分单株收获。M_2代:每一处理取50株 M_1植株评价抗稻胡麻斑病的突变。每株取50粒种子随机混合, Pusa-33, a high-yielding rice variety, is susceptible to rice leaf spot caused by Helmintborium oryzae and therefore has a great loss of grain yield and quality. This experiment attempted to induce resistance mutations by treatment with ethyl methanesulfonate (EMS). M_1 Generation: Seeds of the rice variety Pusa-33 were pre-soaked in distilled water for 12 hours and then treated with 0.15, 0.30, 0.45, 0.60% EMS in pH 5.0, 7.0 and 9.0 for 6 hours at 24 ± 1 ° C. Each treatment and control of the 100 seeds sown in the field. M_1 plants and control plants were harvested at maturity. M_2 generation: Fifty M_1 plants were selected for each treatment to evaluate the resistance to rice mosaic spot disease. Take 50 seeds per plant randomly mixed,