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目的:观察内皮素-1(endothelin-1,ET-1)对人眼小梁细胞细胞骨架肌动蛋白的影响。方法:培养3代人眼小梁细胞,随机分为4组:对照组(0mol/L ET-1);ET-1低剂量组(10-9mol/L ET-1);ET-1中剂量组(10-8mol/L ET-1);ET-1高剂量组(10-7mol/L ET-1),各组细胞分别处理72h后,应用Western-blot方法检测小梁细胞骨架肌动蛋白(F-actin)的表达,并用FITC-phalloidin荧光探针观察肌动蛋白在细胞内的分布。结果:ET-1作用后人小梁细胞肌动蛋白表达明显增高,在10-9～10-7mol/L浓度范围内呈剂量依耐性,荧光探针示ET-1处理组小梁细胞肌动蛋白应力纤维和周边肌动蛋白明显增多浓集,细胞微丝附着与胞膜,细胞间连接及细胞贴壁部位连接明显增多。结论:ET-1能促进小梁细胞肌动蛋白表达,参与了小梁细胞骨架的重构。
Objective: To observe the effect of endothelin-1 (ET-1) on cytoskeleton actin in human trabecular meshwork cells. METHODS: Three generations of human trabecular meshwork cells were cultured and randomly divided into 4 groups: control group (0mol / L ET-1), ET-1 low dose group (10-9mol / L ET-1) (10-8mol / L ET-1) and ET-1 high dose group (10-7mol / L ET-1). The cells in each group were treated for 72h, Western blotting was used to detect the actin (F-actin) expression, and FITC-phalloidin fluorescence probe actin distribution in the cell. Results: The ET-1 expression in trabecular meshwork cells was significantly increased in a dose-dependent manner in the concentration range of 10-9 ~ 10-7mol / L. Fluorescent probes showed that the ET-1-treated trabecular meshwork cells Protein stress fibers and peripheral actin increased significantly, cell microfilament attachment and membrane, cell junctions and cell attachment sites significantly increased. Conclusion: ET-1 can promote the actin expression of trabecular meshwork cells and participate in the reconstruction of trabecular bone cytoskeleton.