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目的:观察长链非编码RNA Crnde通过调节Wnt/β-连环蛋白(β-catenin)信号通路对小鼠成骨细胞增殖的影响。方法:培养MC3T3-E1成骨细胞，随后诱导分化3周，实时定量反转录聚合酶链反应(RT-qPCR)检测Crnde表达，应用CRISPR-Cas9方法构建Crnde基因敲除小鼠模型，同时以野生型小鼠作为对照，分为Crnde基因敲除小鼠组(n n＝25)和野生型小鼠组(n n＝25)，小鼠处死前12 h腹腔注射BrdU进行染色观察成骨细胞增殖，检测血清1型前胶原N末端(P1NP)和Ⅰ型胶原羧基端交联肽(CTX-Ⅰ)表达，蛋白质印迹法(Western blot)检测骨组织Wnt和β-catenin蛋白表达，原位缺口末端标记法(TUNEL)染色检测细胞凋亡情况，分别运用Osteomeasure系统和MicroCT分析进行骨组织形态计量分析，双荧光素酶报告基因验证Crnde与Wnt蛋白的靶向调节关系。组间比较采用n t检验。n 结果:成骨细胞分化过程Crnde表达情况为在诱导的3周时间内，随着时间延长，Crnde表达明显增加，在第3周表达最高(n P>0.05)；Wnt和β-catenin在Crnden -/-小鼠和正常小鼠中表达情况中，蛋白质印迹法(Western blot)结果显示，Crnden -/-组中Wnt和β-catenin蛋白表达低于WT小鼠组(n P0.05)；μCT和Osteomeasure系统结果显示，椎骨的组织形态分析BV/TV，WT组[(19.43±1.53)%]，Crnden -/-组[(20.14±1.56)%]，Crnden -/-鼠的小梁和皮质骨均显示出低骨量表型，Crnden -/-小鼠的骨形成参数均与野生型小鼠差异有统计学意义(n P0.05). The expression of Wnt-catenin and β-catenin protein in the CRNDEn -/-mice decreased significantly as compared with wild-type normal mice (n P0.05). CT and Osteomeasure system showed that the morphological analysis of vertebrae BV/TV was (19.43±1.53)% in WT group and (20.14±1.56)% in CRNDEn -/- group. The trabecular and cortical bone of Crnden -/-mice showed a low bone mass phenotype, and the bone formation parameters of Crnden -/-mice were significantly different from those of wild-type mice (n P<0.05). Serum P1NP and CTX-I in CRNDEn -/- mice were (15.62±1.23) and (12.36±1.12) μg/L, respectively. Serum P1NP and CTX-I in WT mice were (22.81±1.56) and (16.21±1.21) μg/L, respectively. The serum P1NP and CTX-I levels in Crnde n -/-mice were significantly lower than those in wild-type mice (n P<0.05). The results of double luciferase reporter gene assay showed that Crnde mimics significantly decreased the luciferase activity in the wild-type group (n P0.05).n Conclusion:Crnde regulates bone formation through Wnt/-catenin signaling.