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目的:阐明大青叶的中枢解热机制,为其临床应用提供实验依据和理论指导。方法:实验用新西兰兔复制白介素-1β(interleukin-1β,IL-1β)发热模型,在观察大青叶注射液(Daqingyeinjection,DQYI)解热作用的基础上,应用原位杂交技术检测静脉注射DQYI对IL-1β作用下新西兰兔视前区-下丘脑前部(preopticanteriorhypothalamus,POAH)组织中前列腺素E3受体(E-typeprostaglandinreceptor,EP3)mRNA表达的影响。结果:(1)DQYI静脉注射后对正常新西兰兔结肠温度没有明显影响,与生理盐水组相比,最大体温上升高度(△T)及1h体温反应指数(TRI1)两组间无统计学意义(P>0.05);IL-1β组在静脉注射IL-1β后,结肠温度逐渐升高,△T及TRI1与生理盐水组相比,也有统计学意义(P<0.01);而DQYI+IL-1β组结肠温度上升峰值明显低于IL-1β组,两组△T及TRI1比较,有显著性差异(P<0.05)。(2)新西兰兔下丘脑POAHEP3mRNA在生理状态下仅有少量或没有表达;IL-1β诱导发热后,EP3mRNA表达明显增强;而DQYI能减少IL-1β作用下EP3mRNA的表达(P<0.05)。结论:DQYI对IL-1β诱导的新西兰兔发热有解热作用;且其解热作用机制可能与其抑制下丘脑EP3mRNA的表达有关。
Objective: To clarify the central antipyretic mechanism of Daqingye, and provide experimental basis and theoretical guidance for its clinical application. METHODS: New Zealand rabbits were used to replicate the interleukin-1β (IL-1β) fever model. Based on the observation of the antipyretic effects of Daqingyeinjection (DQYI), intravenous DQYI was detected by in situ hybridization. The effects of IL-1β on the expression of prostaglandin E3 receptor (EP3) mRNA in the preoptic hypopothalamus (POAH) tissue of New Zealand rabbits. Results: (1) DQYI had no significant effect on the colon temperature of normal New Zealand rabbits after intravenous injection. There was no significant difference between the two groups in maximal body temperature elevation (△T) and 1h body temperature response index (TRI1). P>0.05); IL-1β group after the intravenous injection of IL-1β, the colon temperature gradually increased, △ T and TRI1 compared with the saline group, also had statistical significance (P <0.01); and DQYI + IL-1β The peak temperature of the colon in the group was significantly lower than that of the IL-1β group. There was a significant difference between the two groups in △T and TRI1 (P<0.05). (2) POAHEP3 mRNA in the hypothalamus of New Zealand rabbits showed only little or no expression under physiological conditions. After IL-1β induced fever, EP3 mRNA expression was significantly increased, while DQYI could reduce the expression of EP3 mRNA under IL-1β (P<0.05). CONCLUSION: DQYI has antipyretic effects on fever induced by IL-1β in New Zealand rabbits, and its antipyretic mechanism may be related to its inhibition of hypothalamic EP3 mRNA expression.