论文部分内容阅读
目的利用抗原抗体反应与培养法结合,建立先分离再增菌的增菌方法,提高志贺菌的培养检出率。方法将包被了志贺菌多价诊断血清的硝酸纤维素膜(NC膜)固定于拭子上,将拭子插入待测样本37℃孵育30 min,取出冲洗后,置新的营养肉汤管中再继续37℃增菌3 h,冲洗后将拭子涂在SS培养基平板上37℃培养18 h~24 h,计数阳性菌落数。结果通过对随机PCR法筛查阳性临床标本的检验,与传统方法相比较,用NC膜富集法增菌3 h,其检出志贺菌菌落的数目显著增高(t=2.56,P<0.05)。结论此方法大大提高了传统培养法的检出率以及增菌环节的选择性,减少了杂菌竟争性抑制和干扰。
OBJECTIVE: To establish the method of adding bacteria that separated first and then add bacteria to improve the detection rate of Shigella culture by combining antigen-antibody reaction with culture method. Methods The nitrocellulose membrane (NC membrane) coated with multivalent diagnostic serum of Shigella was immobilized on the swab. The swab was inserted into the sample to be tested and incubated for 30 min at 37 ℃. After being washed out, a new nutrient broth Tubes and then continue to 37 ℃ enrichment 3 h, after washing the swab coated SS medium plate 37 ℃ cultured 18 h ~ 24 h, counting the number of positive colonies. Results Compared with the traditional methods, the number of colonies detected by random PCR method was significantly increased (t = 2.56, P <0.05) by NC membrane enrichment for 3 h ). Conclusion This method greatly improves the detection rate of the traditional culture method as well as the selectivity of the increased bacteria, reducing the competitive inhibition and interference of bacteria.