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以往研究紫菜细胞对抗生素的敏感性时,常以叶状体的酶解单离体细胞为材料,但由于来自同一个叶状体的体细胞处于不同的分化阶段,离体培养时,体细胞的成活、再生和发育途径均不相同,从而无法准确地判断抗生素对紫菜离体细胞的生长和发育影响。以成活、萌发和发育途径等几乎一致的条斑紫菜壳孢子为研究材料,较系统地观察了3种抗生素对壳孢子的成活、生长和发育的影响。结果表明:卡那霉素能促进条斑紫菜壳孢子的萌发、生长和发育。加卡那霉素培养7d,26%的壳孢子萌发体,其细胞数多于20个,比对照组多2倍,培养11d和18d,萌发体的平均长度分别为对照组的1.6倍和2.1倍。氨苄青霉素能显著地抑制条斑紫菜的壳孢子萌发、生长和发育,造成细胞的分裂速度减缓、叶状体发育异常。加氨苄青霉素培养7d,80%的壳孢子萌发体的细胞数少于15个,培养11d和18d,各试验组的壳孢子萌发体的平均长度均显著低于对照组,并且随着氨苄青霉素浓度的提高,萌发体的平均长度下降。氯霉素对条斑紫菜的壳孢子具有强烈的致死作用。加氯霉素培养7d,浓度大于100μg/mL的各试验组的壳孢子萌发体全部死亡;培养11d,低浓度组(50μg/mL)的萌发体也全部死亡,90%以上的萌发体的细胞数小于5个。由此得出,氯霉素是开展条斑紫菜基因工程的理想抗性选择压力。
In the past to study the sensitivity of seaweed cells to antibiotics, often to the fronds of enzymatic dissociation of single cells as material, but from the same leaf body somatic cells at different stages of differentiation, in vitro culture, somatic cells Survival, regeneration and developmental pathways are not the same, which can not accurately determine the antibiotic on seaweed isolated cells in vitro growth and development. The almost identical Porphyra yezoensis, such as viable, germination and developmental pathways, were used as materials to systematically observe the effects of three antibiotics on the survival, growth and development of conchospores. The results showed that kanamycin can promote the germination, growth and development of Porphyra yezoensis. After 7 and 26 days of ganakamycin cultivation, the number of sporoderm cells was more than 20, more than 2 times of that of the control group. The average length of germinated plants was 1.6 and 2.1 times higher than that of the control group Times Ampicillin can significantly inhibit the germination, growth and development of P. echinula conchospores, resulting in slow cell division and abnormal frond development. After cultured with ampicillin for 7 days, the number of cells in 80% of conchospore germination was less than 15, and the average length of conchospore germination in each experimental group was significantly lower than that of control group on the 11th and 18th day. With the increasing of ampicillin concentration The average length of germination decreased. Chloramphenicol has a strong lethal effect on the conchospores of Porphyra yezoensis. All the test conchospores germinated at the concentration of more than 100μg / mL for 7d after chloramphenicol all died. At 11d, all the germinated ones in the low concentration group (50μg / mL) died, and more than 90% Number less than 5. It is concluded that chloramphenicol is the ideal choice of resistance to carry out genetic engineering of Porphyra yezoensis.