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应用人胎盘型谷胱甘肽-S转移酶(GST-π)抗体,以免疫组化PAP法检测85例大肠癌、34例腺瘤、9例腺瘤癌变组织GST-π活性。结果显示:27例正常大肠粘膜GST-π均呈阴性。大肠癌组织中GST-π呈阳性者74例,总阳性率为87.05%;绒毛状腺癌和高分化管状腺癌阳性率为93.33%,未分化癌和粘液腺癌阳性率只达77.27%。视为癌前疾病的异型增生腺瘤34例,总阳性率为64.7%,轻度和中～重度异型增生阳性率分别为42.85%和80%,腺瘤癌变9例为阳性。结果表明GST-π抗体免疫组化的检测,可作为大肠癌早期诊断一项新的较灵敏的指标。GST-π在大肠癌组织中的含量及分布除标志分泌、释放的不同外,可能有助于判断大肠癌的预后。
Human placental glutathione-S transferase (GST-π) antibody was used to detect GST-π activity in 85 cases of colorectal cancer, 34 cases of adenoma, and 9 cases of adenoma with immunohistochemical PAP. The results showed that: 27 cases of normal colorectal mucosa were negative for GST-π. The positive rate of GST-π in colorectal cancer tissues was 74. The total positive rate was 87.05%. The positive rate of villous adenocarcinoma and highly differentiated tubular adenocarcinoma was 93.33%. The positive rate of undifferentiated carcinoma and mucinous adenocarcinoma was only 77.27%. There were 34 dysplasia adenomas precancerous lesions with a total positive rate of 64.7%. The positive rates of mild to moderate to severe dysplasia were 42.85% and 80%, respectively, and 9 of the adenomas were positive. The results showed that the detection of GST-π antibody immunohistochemistry can be used as a new sensitive indicator for the early diagnosis of colorectal cancer. The content and distribution of GST-π in colorectal cancer tissues may be helpful for judging the prognosis of colorectal cancer, in addition to the differences in secretion and release of markers.