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已知重水(D2O)能增加某些病毒的热稳定性,但其机理不清.用基质辅助气体同位素质谱法,研究了日本乙型脑炎病毒(Japanese encephalitis virus,JEV)和甲型肝炎病毒(hepatitis A virus,HAV)用D2O处理后,测定病毒及其RNA结构中的氘氢比值(D/H),以δDSMOW表示.结果说明,与正常病毒相比,上述D2O处理后的病毒样品中国δDSMOW值明显升高,其升高值与病毒样品的加入量呈良好的线性关系.实验还指出,D2O处理后的病毒国δDSMOW值的升高与其热稳定性的升高是相关联的.实验结果证明,D2O处理导致病毒及其RNA结构中发生氢氘置换,氢氘置换可导致病毒生物学性状(热稳定性增加)的表型改变.
It is known that heavy water (D2O) can increase the thermal stability of some viruses, but its mechanism is unclear.Jean encephalitis virus (JEV) and Hepatitis A virus (D / H) ratio of deuterium hydrogen in virus and its RNA structure after treated with D 2 O with hepatitis A virus (HAV), which is expressed as δDSMOW.The results showed that compared with the normal virus, the above D 2 O-treated virus samples China δDSMOW value was significantly increased, and the value of the increase was linearly correlated with the amount of virus sample added.The experiment also pointed out that the increase of δDSMOW value of virus virus after D2O treatment is related to the increase of its thermal stability.Experiment The results demonstrate that D2O treatment results in hydrogen-deuterium substitutions in the virus and its RNA structure, and that hydrogen-hydrogen-displacement results in a phenotypic change in the biological properties of the virus (increased thermal stability).