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A robust tuning method for (A)str(o)ms two-degree of Freedom Modified Smith Predictor (MSP) is proposed, and it can achieve the fast setpoint and load disturbance responses independently. The tuning rules may meet the requirements within a certain delay margin according to the robusmess theory. The simulation results illustrate that the tuning method is efficient compared with other controllers. Finally, the MSP controller is applied to the 15L batch fermentation system for controlling the temperature in bioreactor, and the experiment results further verify that the robust tuning rules may achieve good performance.