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目的通过豚鼠致敏最大值试验(guinea pig maximization test,GPMT)方法建立豚鼠致敏模型,检测并比较TCE致敏豚鼠与未致敏豚鼠血清中IL-6和IL-8的水平,探讨IL-6和IL-8在三氯乙烯(TCE)过敏性皮炎中的作用。方法将豚鼠随机分为空白对照组,溶剂(橄榄油)对照组,DNCB阳性对照组和TCE处理组。采用GPMT法建立豚鼠致敏模型。根据致敏结果及末次激发后采血的不同时点将TCE处理组分为TCE未致敏24h组,TCE致敏24h组,TCE未致敏72h组,TCE致敏72h组。用ELISA试剂盒测定血清中IL-6和IL-8的含量。结果DNCB组致敏率为100%,TCE组致敏率为62.1%。溶剂对照组与空白对照组差异均无统计学意义。与溶剂对照组相比,TCE未致敏24h组IL-6水平降低,差异有统计学意义(P<0.05),TCE未致敏72h组与TCE未致敏24h组相比,IL-6水平明显升高,差异有统计学意义(P<0.05)。与溶剂对照组相比,TCE未致敏72组IL-8水平明显降低,差异有统计学意义(P<0.05)。结论血清中细胞因子IL-6和IL-8水平在TCE诱导的致敏组和未致敏组豚鼠间,仅个别时间段的差异有统计学意义,提示两者可能仅在一定时间段中发挥作用。
Objective To establish the guinea pig sensitization model by guinea pig maximization test (GPMT) and to detect and compare the levels of IL-6 and IL-8 in serum of TCE sensitized guinea pigs and non-sensitized guinea pigs, 6 and IL-8 in trichlorethylene (TCE) atopic dermatitis. Methods Guinea pigs were randomly divided into blank control group, solvent (olive oil) control group, DNCB positive control group and TCE treatment group. Guinea pig sensitization model was established by GPMT method. According to the results of sensitization and different time after the last challenge, the TCE group was divided into TCE non-sensitized 24h group, TCE sensitized 24h group, TCE unsensitized 72h group and TCE sensitized 72h group. Serum IL-6 and IL-8 levels were measured by ELISA kit. Results The sensitization rate was 100% in DNCB group and 62.1% in TCE group. Solvent control group and the blank control group differences were not statistically significant. Compared with the solvent control group, the level of IL-6 in TCE non-sensitized group was significantly lower than that in control group (P <0.05) Was significantly higher, the difference was statistically significant (P <0.05). Compared with the solvent control group, the level of IL-8 in TCE non-sensitized 72 group was significantly lower (P <0.05). Conclusion The levels of cytokines IL-6 and IL-8 in serum are statistically different only in individual time periods between TCE-induced sensitized group and non-sensitized group of guinea pigs, suggesting that both may only play a certain period of time effect.