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目的研究美藤果油对非酒精性脂肪性肝炎(NASH)大鼠的保护作用。方法采用高脂饲料饲喂SD大鼠4周,建立NASH模型,设置空白组,NASH模型组,脂肪性肝炎对照组,紫苏油对照组,美藤果油高(1.6 m L/kg)、中(0.8 m L/kg)、低(0.4 m L/kg)剂量组,每组12只大鼠。给药45 d后,检测各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平,并使用HE染色法观察比较各组的肝脏病理情况,进行统计比较。结果模型组的TC、TG、LDL-C水平依次为(1.295±0.143)、(0.738±0.095)、(0.194±0.019)mmol/L;美藤果油中剂量组的TC、TG、LDL-C水平依次为(0.610±0.031)、(0.402±0.020)、(0.136±0.005)mmol/L;美藤果油高剂量组的TC、TG、LDL-C水平依次为(0.662±0.036)、(0.436±0.029)、(0.147±0.011)mmol/L;相对于模型组,美藤果油中、高剂量组的TC、TG、LDL-C水平均下降(P<0.05或<0.01)。相对于模型组,美藤果油中、高剂量组对ALT、AST、TNF-α、IL-6水平均有下调作用(P<0.05或<0.01),且对NASH大鼠的肝脏病理改变有改善作用(P<0.01)。结论美藤果油对NASH大鼠具有一定的保护作用,可能主要通过降低血脂水平和抑制炎症细胞因子而实现。
Objective To study the protective effect of sodium rattan oil on non-alcoholic steatohepatitis (NASH) rats. Methods High fat diet was used to feed SD rats for 4 weeks. NASH model was established. The blank control group, NASH model group, steatohepatitis control group, perilla oil control group, Medium (0.8 m L / kg) and low (0.4 m L / kg) dose groups, 12 rats in each group. After 45 d of administration, serum TC, TG, HDL-C, LDL-C, alanine (ALT), aspartate aminotransferase (AST), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were measured. The pathological changes of liver were observed by HE staining , For statistical comparison. Results The levels of TC, TG and LDL-C in the model group were (1.295 ± 0.143), (0.738 ± 0.095) and (0.194 ± 0.019) mmol / L, The levels of TC, TG and LDL-C in high-dose group were (0.662 ± 0.036), (0.436 ± 0.036) and ± 0.029) and (0.147 ± 0.011) mmol / L, respectively. Compared with the model group, the levels of TC, TG and LDL-C in the middle and high dose groups decreased significantly (P <0.05 or <0.01). Compared with the model group, METT medium and high dose groups had down-regulated ALT, AST, TNF-α and IL-6 levels (P <0.05 or <0.01) Improve the effect (P <0.01). Conclusion Methyl rapamycin has a protective effect on NASH rats, which may be mainly achieved by lowering blood lipid levels and inhibiting inflammatory cytokines.