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目的探讨姜黄素对甲状腺未分化癌阿霉素耐药细胞株HTh74Rdox的增殖抑制作用及可能的作用机制。方法以不同浓度阿霉素处理甲状腺未分化癌细胞株HTh74及阿霉素耐药株HTh74Rdox细胞48h后,MTT法检测细胞活力,计算两种细胞的半数抑制浓度(IC50)和HTh74Rdox细胞的阿霉素耐药指数。不同浓度姜黄素处理HTh74及HTh74Rdox细胞,24h后倒置显微镜下观察细胞形态的变化,并采用MTT法检测细胞活力。Western blot检测不同浓度姜黄素处理HTh74Rdox细胞6h后p-mTOR、p-S6K和p-Akt蛋白表达的变化。结果阿霉素对HTh74细胞的IC50为(148.77±17.47)ng/ml;而对HTh74Rdox细胞的IC50则为(2011.00±109.42)ng/ml。HTh74Rdox细胞对阿霉素的耐药指数为13.59±0.98。在姜黄素作用下,HTh74及HTh74Rdox细胞形态均发生改变,细胞活力也均被抑制(P<0.01),但HTh74Rdox细胞对姜黄素更为敏感。不同浓度姜黄素处理HTh74Rdox细胞6h后,p-mTOR、p-S6K和p-Akt蛋白表达均受到抑制(P<0.01),且呈现明显的剂量依赖性。结论与HTh74细胞相比,对阿霉素耐药的HTh74Rdox细胞对姜黄素更为敏感,这可能与姜黄素能够显著抑制HTh74Rdox细胞中p-mTOR、p-S6K和p-Akt蛋白表达有关。
Objective To investigate the inhibitory effect of curcumin on the proliferation of doxorubicin-resistant cell line HTh74Rdox and its possible mechanism. Methods Thyrotropin (HTh74) and doxorubicin-resistant (HTh74Rdox) cells were treated with doxorubicin at different concentrations for 48 h. MTT assay was used to determine the half-inhibitory concentration (IC50) and HTh74Rdox Su-resistant index. HTh74 and HTh74Rdox cells were treated with different concentrations of curcumin, and the morphological changes of cells were observed under inverted microscope 24 hours later. Cell viability was measured by MTT assay. The expression of p-mTOR, p-S6K and p-Akt protein in HTh74Rdox cells treated with different concentrations of curcumin were detected by Western blot. Results The IC50 of doxorubicin to HTh74 cells was (148.77 ± 17.47) ng / ml and that of HTh74Rdox cells was (2011.00 ± 109.42) ng / ml. The resistance index of doxorubicin to HTh74Rdox cells was 13.59 ± 0.98. Under the action of curcumin, the morphology of HTh74 and HTh74Rdox cells were changed, and cell viability was also inhibited (P <0.01), but HTh74Rdox cells were more sensitive to curcumin. The expressions of p-mTOR, p-S6K and p-Akt protein in HTh74Rdox cells treated with different concentrations of curcumin for 6 h were significantly inhibited (P <0.01) and showed a dose-dependent manner. CONCLUSION: Adriamycin-resistant HTh74Rdox cells are more sensitive to curcumin than HTh74 cells, which may be related to the fact that curcumin can significantly inhibit the expression of p-mTOR, p-S6K and p-Akt in HTh74Rdox cells.