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选择巯基化并含有PW17碱基序列的DNA在金电极上自组装,在10μmol/L Pb2+存在下,Pb2+诱导自组装的DNA形成Pb2+稳定的G-四联体.通过微分脉冲伏安法发现Pb2+稳定的G四联体在-0.365 V vs.Ag/AgCl出现一Pb2+的还原峰.依据Pb2+与EDTA的强配位作用,EDTA可以与G-四联体中的Pb2+作用,并伴随G-四联体的构型转变为自由态的DNA.以组装在电极上的DNA为工作单元,Pb2+和EDTA作为输入信号,-0.365 V处的还原峰为输出信号,根据Pb2+和EDTA加入顺序的不同,键盘锁处于“开”或“关”的状态.在Pb2+与EDTA的交替作用下,G-四联体和自由态DNA可以互相转化,同时电化学的输出信号在循环5次后基本保持不变,键盘锁呈现出良好的可重置性.
The thiolated DNA sequence containing PW17 was self-assembled on gold electrode, and Pb2 + -induced self-assembled DNA was formed into Pb2 + stable G-quadruplex in the presence of 10μmol / L Pb2 + by differential pulse voltammetry. Pb2 + The stable G quadruplex exhibits a reduction peak of Pb2 + at -0.365 V vs. Ag / AgCl.According to the strong coordination between Pb2 + and EDTA, EDTA can interact with Pb2 + in the G-quadruplex with G-tetrakis The configuration of the conformational body is transformed into free-form DNA. With the DNA assembled on the electrode as the working unit, Pb2 + and EDTA are taken as the input signals and the reduction peak at -0.365 V is the output signal. According to the order of adding Pb2 + and EDTA, The keyboard lock is in the state of “on” or “off.” Under the action of Pb2 + and EDTA, the G-quadruplex and free DNA can transform into each other, and the electrochemical output signal is circulated 5 times The basic remains the same, the keyboard lock shows a good resettability.