目的鉴定分析药品中分离的大肠埃希菌可疑菌。方法参照中国药典等文献,采用VITEK微生物鉴定仪对其鉴定,同时采用RiboPrinter全自动微生物基因指纹鉴定系统对其进行鉴定和溯源。结果可疑菌与大肠埃希菌生化特性相符,限制性内切酶EcoR I的酶切图谱与RP数据库中的大肠埃希菌一致,并且与标准菌株相似。结论 RiboPrinter分型结果和分子溯源功能能有效的应用于药品可疑菌的检验。 Objective To identify and analyze the suspected Escherichia coli isolated from drugs. Methods Reference to the Chinese Pharmacopoeia and other documents, using VITEK microbial identification of its identification, and the use of RiboPrinter automatic microbial gene fingerprint identification system for its identification and traceability. Results Susceptible bacteria were consistent with the biochemical characteristics of Escherichia coli. The restriction endonuclease EcoR I digestion map was consistent with that of Escherichia coli in the RP database and similar to the standard strains. Conclusion RiboPrinter typing results and molecular traceability can be effectively applied to the detection of drug suspicious bacteria.