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目的确定2007年陕西省赴柬埔寨务工人员归国后发热疫情病因。方法采集陕西省70例赴柬埔寨务工归国人员的血清标本,使用血清学方法进行登革病毒IgM和IgG检测;使用逆转录-巢式-聚合酶链反应(RT-nPCR)扩增发热患者血清中登革病毒RNA;同时,对发热患者血清进行登革病毒的分离培养。结果使用CORTEZ公司快检试剂共检出7份IgM阳性血清,其中发热患者5例;使用IBL公司IgM试剂盒共检出6份IgM阳性血清,其中发热病例4例。使用IBL公司登革热IgG ELISA试剂盒共检出13份IgG阳性血清,包括2例发热患者。使用RT-nPCR方法从首例发热患者的血清中扩增出登革病毒目的片段,经序列比对结果证实是DEN-1型病毒核酸片段。用C6/36细胞和BHK细胞未分离出登革病毒。结论根据患者的临床症状、流行病学史以及实验室诊断结果,共确诊了4例输入性登革热病例,其中首发病例为DEN-1型病毒感染。血清学检测结果表明,在归国人员中还存在隐性感染者。
Objective To determine the cause of fever after returning to Cambodia in 2007 for migrant workers from Shaanxi Province. Methods A total of 70 serum samples from returnees in Cambodia were collected from Shaanxi province. Serological methods were used to detect dengue virus IgM and IgG. The serum of patients with fever was amplified by reverse transcription-nested polymerase chain reaction (RT-nPCR) Dengue virus RNA; the same time, the fever of the serum dengue virus isolation and culture. Results A total of 7 IgM positive sera were detected using CORTEZ Fast Test Reagent, of which 5 were fever. Six IgM positive sera were detected using the IBM IgM kit, of which 4 were fever. A total of 13 IgG-positive sera were detected using the IBL dengue IgG ELISA kit, including 2 patients with fever. The target fragment of dengue virus was amplified from the serum of the first patients with fever by RT-nPCR. The result of sequence alignment confirmed that it was a DEN-1 type virus nucleic acid fragment. Dengue virus was not isolated from C6 / 36 cells and BHK cells. Conclusions According to the patient’s clinical symptoms, epidemiological history and laboratory diagnosis results, a total of 4 cases of imported dengue were confirmed, of which the first case was DEN-1 type virus infection. Serological test results show that there are still hidden in the return of staff.