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目的:观察连花清瘟胶囊(LHQW)对金黄色葡萄球菌生物膜(S.a BF)形成的抑制作用。方法:微量稀释法检测LHQW最小杀菌浓度(MBC);用结晶紫(CV)和微生物活性(WST)检测法观察LHQW对S.a BF形成的抑制作用;用扫描电镜(SEM)和共聚焦显微镜(CLSM)观察S.a BF形态并测量厚度。结果:LHQW对S.a的MBC为20mg/ml。LHQW可有效抑制S.a BF形成,杀灭膜内细菌;与空白对照组比较,LHQW显著降低S.a BF厚度(P<0.01)。结论:LHQW抑制S.a BF的形成,其评估体系可用于传统中药抗菌作用研究。
Objective: To observe the inhibitory effect of Lianhua Qingwen Capsule (LHQW) on the formation of S.aureus biofilm (S.a BF). Methods: The minimum bactericidal concentration of LHQW (MBC) was detected by the method of microdilution. The inhibitory effect of LHQW on the formation of Sa BF was observed by the method of crystal violet (CV) and microbial activity assay (WST). Scanning electron microscopy (SEM) and confocal microscopy ) Observe the Sa BF morphology and measure the thickness. Results: The MBC of LHQW to S.a was 20 mg / ml. LHQW can effectively inhibit S.a BF formation and kill bacteria in the membrane. Compared with the blank control group, LHQW significantly decreased the thickness of S.a BF (P <0.01). Conclusion: LHQW inhibits the formation of S. a BF. The evaluation system can be used to study the antimicrobial effect of traditional Chinese medicine.