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目的研究饮水添加不同水平硼对大鼠血液成份的影响。方法192只(28±2)天清洁级SD大鼠,随机分为6组,每组32只,雌雄各半,分别饮用添加硼0、40、80、160、320和640mg/L的蒸馏水,试验期60天。分别于试验的第30天和60天每组随机取鼠16只,雌雄各8只,10%水合氯醛腹腔麻醉,右心房采血,测定红白细胞数、网织红细胞数、血红蛋白含量;数制作血涂片,瑞氏-吉姆萨氏染色,进行白细胞分类计数。结果各试验组的网织红细胞数不同程度升高;硼40、80mg/L组的红细胞数和血红蛋白含量多显著升高(P<0.05),各种白细胞比例变化多不显著;硼160～640mg/L组的红、白细胞数和血红蛋白含量均显著或极显著降低(P<0.05或P<0.01),试验第60天大鼠的中性粒细胞、嗜酸性粒细胞、嗜碱性粒细胞和单核细胞比例多显著降低(P<0.05或P<0.01)。结论饮水添加硼40mg/L对大鼠血细胞的发生和血红蛋白的合成均有明显的促进作用;添加硼80mg/L对大鼠红细胞生成和血红蛋白合成有一定促进作用;添加硼160～640mg/L对大鼠血细胞的发生和血红蛋白的合成均有明显的不良影响,甚至具有明显的毒性作用。
Objective To study the effects of adding different levels of boron in drinking water on the blood components in rats. Methods 192 SD rats (28 ± 2 days) were randomly divided into 6 groups (32 rats in each group), male and female rats were drank respectively with 0, 40, 80, 160, 320 and 640 mg / The test period of 60 days. On the 30th day and the 60th day of the experiment, 16 rats were randomly selected in each group, 8 in each male and 8 female, in which 10% chloral hydrate was injected intraperitoneally and blood was taken from the right atrium to determine the number of red and white cells, reticulocytes and hemoglobin. Blood smears, Wright - Jim Sa stain, white blood cell count. Results The numbers of reticulocytes in different experimental groups increased to different extents. The numbers of erythrocyte and hemoglobin in 40, 80 mg / L group were significantly increased (P <0.05) / L group of red and white blood cell count and hemoglobin content were significantly or very significantly reduced (P <0.05 or P <0.01), the test on the 60th day of neutrophil, eosinophil, basophil and The proportion of monocytes decreased significantly (P <0.05 or P <0.01). Conclusion Adding boron 40 mg / L in drinking water can significantly promote the formation of hemocytes and the synthesis of hemoglobin in rats. Adding boron 80 mg / L can promote erythropoiesis and hemoglobin synthesis in rats. Adding boron 160 ~ 640 mg / L Rat blood cells and hemoglobin synthesis have obvious adverse effects, and even have obvious toxic effects.