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目的:建立检测止咳中药制剂中非法掺入的化学止咳平喘药物沙丁胺醇方法。方法:选用Agilent C18(4.6 mm×150 mm,5μm),以甲醇-磷酸二氢钾溶液(用磷酸调节pH 3.0)5∶95为流动相。根据所检测到化合物的色谱保留时间,并与对照品比较,鉴别中药制剂中非法掺入的沙丁胺醇。结果:本实验建立的条件能够把中药制剂中添加的化学药物(沙丁胺醇)完全分离开来;对20个制剂样品用建立的分析技术进行了检测,经与相应的对照品比较,20个样品均未检测到沙丁胺醇。结论:该方法选择性强,灵敏度高,可作为中药制剂中非法加入掺入化学药物成分沙丁胺醇的有效检测手段。
Objective: To establish a method for testing salbutamol, a chemical cough and antiasthmatic drug, illegally incorporated into traditional Chinese medicine preparations for relieving cough. METHODS: Agilent C18 (4.6 mm × 150 mm, 5 μm) was used as the mobile phase with methanol-potassium dihydrogen phosphate solution (adjusted to pH 3.0 with phosphoric acid) 5:95. Based on the chromatographic retention times of the compounds detected, and compared with the control, albuterol illegally incorporated into the Chinese traditional medicine formulation was identified. Results: The conditions established in this experiment could completely separate the chemical drugs (albuterol) added in the traditional Chinese medicine preparation. Twenty tissue samples were tested by the established analytical techniques. Compared with the corresponding reference materials, all 20 samples Salbutamol was not detected. Conclusion: This method is highly selective and sensitive, and can be used as an effective detection method for the illegal addition of salbutamol as a chemical component in traditional Chinese medicine preparations.