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目的探讨不同浓度芬太尼对胃癌SGC-7901细胞的影响。方法分别以0、0.5、5、50、500nmol/L芬太尼作用于胃癌SGC-7901细胞24h后,双醋酸荧光素(FDA)/碘化丙啶(PI)荧光染色观察细胞膜的通透性;细胞活力检测试剂盒检测细胞活力;磷脂酰丝氨酸蛋白抗体-异硫氰酸荧光素(Annexin V-FITC)/PI双标记染色后,用流式细胞术检测胃癌细胞的凋亡,细胞周期试剂盒分析胃癌细胞的细胞周期变化。结果不同浓度芬太尼共培养24h后,随着芬太尼的浓度逐渐增加,胃癌细胞死亡数量逐渐增长,存活细胞数量逐渐减少;细胞活力呈下降趋势,芬太尼浓度在50nmol/L时即可产生明显的抑制作用;胃癌SGC-7901细胞细胞周期分析显示,部分细胞阻滞于G2/M期。结论芬太尼可以使胃癌SGC-7901细胞的活力下降,促进胃癌细胞凋亡和/或死亡。
Objective To investigate the effects of different concentrations of fentanyl on gastric cancer SGC-7901 cells. Methods After the cells were treated with 0, 0.5, 5, 50 and 500 nmol / L fentanyl for 24 hours, the permeability of cell membrane was observed by fluorescence staining with FDA / propidium iodide (PI) Cell viability was detected by cell viability assay kit. After the cells were stained with phosphatidylserine protein antibody (Annexin V-FITC) / PI double staining, the apoptosis of gastric cancer cells was detected by flow cytometry. Box analysis of gastric cancer cell cycle changes. Results After 24 hours of co-culture with different concentrations of fentanyl, with the increase of fentanyl concentration, the number of gastric cancer cells gradually increased and the number of surviving cells decreased gradually. The viability of fentanyl cells tended to decrease. When the concentration of fentanyl was 50nmol / L The cell cycle analysis of gastric cancer SGC-7901 cells showed that some cells were arrested in G2 / M phase. Conclusion Fentanyl can decrease the viability of gastric cancer SGC-7901 cells and promote the apoptosis and / or death of gastric cancer cells.