目前用于病毒性肝炎诊断的实验室检验方法主要有病毒抗原、抗体的血清学试验和以PCR为主的病毒核酸测定试验。由于抗原、抗体反应的血清学试验方法的检测灵敏度有一定的局限性,比如血清中病毒抗原的浓度过低,或抗体尚未产生（所谓的“窗口期”）时,会造成检测不出。因此,以PCR为主的核酸扩增技术在填补血清学检验技术不足方面,发挥了一定作用。以丙型肝炎病毒的核酸扩增检测 Currently used for the diagnosis of viral hepatitis laboratory testing methods are mainly viral antigens, antibody serological tests and PCR-based viral nucleic acid assay. The detection sensitivity of antigenic and antibody serological tests has some limitations, such as undetectable levels of viral antigens in serum or undetected antibodies (so-called “window period”). Therefore, the PCR-based nucleic acid amplification technology to fill in the lack of serological testing technology, has played a certain role. Detection of hepatitis C virus nucleic acid amplification