引言真核细胞的基因表达受多种因子或环境信号的调节,其中,外源蛋白质或核酸分子对细胞基因表达的影响日益受到重视。近年来,在细胞融合基础上建立的红细胞介导大分子进入哺乳类细胞的微量注射技术,为研究外源大分子物质在细胞内的功能及其对细胞基因表达的调控提供了有效的手段。它使一些已经在非细胞系统(如分离的染色质系统)得到证实的 INTRODUCTION Gene expression in eukaryotic cells is regulated by a variety of factors or environmental signals. Among them, the influence of foreign proteins or nucleic acid molecules on gene expression of cells is increasingly receiving attention. In recent years, the establishment of red blood cell-mediated microinjection of macromolecules into mammalian cells based on cell fusion provides an effective means for studying the function of exogenous macromolecules in cells and the regulation of cellular gene expression. It allows some of the cells that have been confirmed in non-cellular systems (such as isolated chromatin systems)