目的　检测Hoxd 13基因在肛门直肠畸形病人和正常人的直肠末端表达状况 ,探讨Hoxd 13基因与人类先天性肛门直肠畸形发生的关系。方法　应用RT -PCR方法检测Hoxd 13基因在 16例肛门直肠畸形患儿和 5例正常儿童直肠末端的表达水平。结果　Hoxd 13基因mRNA在先天性无肛畸形直肠末端表达水平明显低于正常直肠末端的表达 (0 .32± 0 .36vs 0 .73± 0 .10 )。 (P <0 .0 1)。结论　Hoxd 13基因表达可能与人类先天性肛门直肠畸形的发生有关 Objective To detect the expression of Hoxd 13 gene in the rectum of patients with anorectal malformations and normal persons and to explore the relationship between Hoxd 13 gene and the development of human congenital anorectal malformations. Methods RT-PCR was used to detect the expression of Hoxd 13 gene in the rectum of 16 children with anorectal malformations and 5 normal children. Results The mRNA expression of Hoxd 13 in the rectum of congenital anorectal malformations was significantly lower than that in the normal rectum (0.32 ± 0.36 vs. 0.73 ± 0.10). (P <0. 01). Conclusion Hoxd 13 gene expression may be related to the occurrence of congenital anorectal malformation