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针对胡麻组织培养中胚状体诱导率低、植株再生和生根困难等问题,以强抗旱胡麻优良新品种陇亚11号为实验材料,从培养基配置、外植体筛选、生根培养等方面开展系统研究,并进行相关技术优化,为胡麻组织培养技术提供一套更加适用的优化方案。结果表明,NAA与6-BA互作,诱导的愈伤组织深绿、致密、团状性好,玻璃化和疏松透明现象较少,二者浓度的变化对胚状体的诱导率平均值和最大值以及成苗率平均值等均高于优化前,其中MS+NAA(1.0mg.L-1)+6-BA(0.5mg.L-1)的培养基胚状体诱导率、成苗率均为所有处理中最高;NAA与PP333互作,生根率、平均生根数、平均根长均有明显的提高,其中1/2MS+AC(0.05%)+NAA(0.5mg.L-1)+PP333(0.001mg.L-1)的培养基生根效果最好,该培养基上培养的试管苗,根上发生较多的侧根和不定根,有利于幼苗吸收土壤中的水分和无机盐,提高移栽成活率。虽然优化后的培养基使胚状体的诱导率和成苗率均有明显增加,但通过胚状体分化的植株比率仍然偏低,与实际生产中的要求还有一定差距,不利于大量的繁殖移栽,仍需进一步探索研究。
In order to solve the problems of low induction rate, plant regeneration and rooting difficulties in flax tissue culture, the excellent new variety “Longya 11” with strong drought tolerance flax was used as the experimental material, including medium configuration, explant screening and rooting culture Systematic research, and related technology optimization, flax tissue culture technology to provide a more suitable optimization program. The results showed that the interactions between NAA and 6-BA induced the dark green calluses and the dense clumps. The vitrification and looseness of NAA were less. The average value of the induction rate of embryoid body and The maximum and the average value of seedling formation rate were higher than those before optimization, including the induction rate of embryoid body of MS + NAA (1.0mg.L-1) + 6-BA (0.5mg.L-1) NAA interacted with PP333, the rooting rate, the average number of rooting and the average root length were significantly increased, of which 1 / 2MS + AC (0.05%) + NAA (0.5mg.L-1) + PP333 (0.001mg.L-1) had the best rooting effect. The test-tube plantlets cultured on this medium had more lateral roots and adventitious roots, which could help the seedlings to absorb the water and inorganic salts in the soil, Survival rate. Although the optimized culture medium increased the induction rate and seedling emergence rate of embryoid bodies, the ratio of plants differentiated by embryoid bodies was still low, which still lags behind the requirements in actual production and is not conducive to a large amount of Breeding transplanting, still need further exploration and research.