论文部分内容阅读
目的通过实时荧光定量聚合酶链反应(FQ-PCR)检测闽南人人乳头状瘤病毒(HPV)感染状况,探讨HPV感染与闽南人食管癌发生的关系。方法采用FQ-PCR技术分别对2009年10月至2012年12月出生生长于闽南的100名健康人[健康组,男66例、女34例,年龄(52.35±6.72)岁]和100例食管鳞癌患者[食管癌组和癌旁组,男64例、女36例,年龄(51.62±6.37)岁]进行HPV-6、HPV-11、HPV-16、HPV-18检测。结果 100例食管鳞癌组织、癌旁组织及100名健康人食管黏膜组织中HPV感染阳性者分别为22例、8例、6例,食管癌组与健康组HPV感染差异有统计学意义(χ2=10.63,P<0.01);食管癌组和癌旁组及健康组的食管黏膜组织中HPV-6、HPV-11、HPV-16、HPV-18的阳性感染者分别为11例、11例、14例、15例和5例、6例、7例、8例及5例、5例、6例、6例,各组间差异无统计学意义(P>0.05);高、中、低分化鳞癌中HPV阳性感染者分别为1例、21例、5例,各组间差异无统计学意义(P>0.05)。结论闽南地区居民食管鳞癌组织中可能存在HPV感染,HPV感染可能与食管鳞癌发生、发展有关。
Objective To detect human papillomavirus (HPV) infection in Minnan by real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) and to explore the relationship between HPV infection and esophageal cancer in Fujian. Methods 100 healthy people (healthy group, 66 males, 34 females, age (52.35 ± 6.72) years old] and 100 cases of esophageal HPV-6, HPV-11, HPV-16 and HPV-18 were detected in patients with squamous cell carcinoma [esophageal cancer group and paracarcinoma group, 64 males and 36 females, aged 51.62 ± 6.37 years. Results The positive rate of HPV infection in 100 cases of esophageal squamous cell carcinoma, paracancer tissue and 100 healthy human esophageal mucosa were 22, 8 and 6 respectively. There was significant difference in HPV infection between esophageal cancer group and healthy group (χ2 = 10.63, P <0.01). The positive rates of HPV-6, HPV-11, HPV-16 and HPV-18 in esophageal cancer group, paracancer group and healthy group were 11 and 11 respectively 14 cases, 15 cases and 5 cases, 6 cases, 7 cases, 8 cases and 5 cases, 5 cases, 6 cases and 6 cases. There was no significant difference between the groups (P> 0.05) There were 1 case of HPV positive in squamous cell carcinoma, 21 cases and 5 cases, respectively. There was no significant difference between the groups (P> 0.05). Conclusions There may be HPV infection in esophageal squamous cell carcinoma in Minnan area. HPV infection may be related to the occurrence and development of esophageal squamous cell carcinoma.