论文部分内容阅读
用SMMC-7721人肝癌细胞株,经10μmol/L维甲酸(RA)处理后,其生长受到抑制,停留在G0/G1期细胞比例上升,而S、G2+M期细胞的比例下降。光镜及图象分析发现该细胞经RA作用后,形态由长棱形为主且核大转变为以多边形为主且核小,核浆比例由0.595下降至0.247。免疫荧光法检测发现,经RA作用后细胞膜上纤维连接蛋白的含量上升。经RA处理的该株细胞,AFP的分泌量明显低于相应天数的对照细胞,而白蛋白的分泌量则明显增高。结果表明:RA可以从细胞生物学、形态学发生物化学水平逆转人肝癌细胞表型。
After SMMC-7721 human hepatocellular carcinoma cell line was treated with 10 μmol/L retinoid acid (RA), its growth was inhibited, the proportion of cells staying in G0/G1 phase increased, and the proportion of cells in S and G2+M phases decreased. Light microscopy and image analysis showed that after the cells were treated with RA, the shape of the cells was mainly long prisms, and the nucleus was changed to be mainly polygonal and the nucleus was small. The proportion of nuclear plasma decreased from 0.595 to 0.247. Immunofluorescence assay showed that the content of fibronectin on the cell membrane increased after RA treatment. In the cells treated with RA, the secretion of AFP was significantly lower than that of the corresponding cells in the corresponding days, and the secretion of albumin was significantly increased. The results showed that RA could reverse the phenotype of human hepatoma cells from the levels of cell biology and morphology.