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目的验证老年大鼠心肌细胞凋亡程度;测定老年心肌组织中内源性线粒体后凋亡调节蛋白Omi/HtrA2和XIAP的表达及其与心肌细胞凋亡的关系。方法分别选取雄性成年SD大鼠[体重(278±10)g,4～6个月]和老年SD大鼠[体重(525±8)g,22～24个月]。随机分为以下4组:正常成年组(40只);正常老年组(40只);老年ucf-101组(10只),腹腔注射ucf-101(1.5μmol/kg);老年DMSO组(10只),腹腔注射DMSO(1.5μmol/kg)。采用Caspase-3活性测定法检测大鼠心肌组织中心肌细胞凋亡发生情况;用West-ern-blot蛋白印记法检测大鼠心肌组织中Omi/HtrA2和XIAP的表达水平。结果以成年大鼠caspase-3比活性(1.00±0.04)为1,老年大鼠的caspase-3的比活性(2.31±0.43,P<0.01)显著增高。老年大鼠心肌组织中Omi/HtrA2表达明显增高,XIAP表达明显下降。给予特异性Omi/HtrA2的抑制剂ucf-101,可显著减少老年大鼠心肌组织caspase-3的表达。结论老年大鼠心肌组织中表达增多的Omi/HtrA2,可能导致了XIAP的降解以及随后的caspase-3活化和细胞凋亡。
Objective To verify the apoptosis of cardiomyocytes in senile rats and to determine the expression of apoptotic regulatory proteins Omi / HtrA2 and XIAP after myocardial mitochondria and their relationship with myocardial apoptosis in elderly myocardial tissues. Methods Male adult SD rats [weighing (278 ± 10) g, 4 to 6 months] and old SD rats [weighing 525 ± 8 g, 22 to 24 months] were selected. The rats were randomly divided into the following 4 groups: normal adult group (40 rats), normal aged group (40 rats), aged ucf-101 group (10 rats) and intraperitoneal injection of ucf-101 Only), intraperitoneal injection of DMSO (1.5μmol / kg). Caspase-3 activity assay was used to detect cardiomyocyte apoptosis in rat myocardium. Expression of Omi / HtrA2 and XIAP in rat myocardium was detected by West-ern-blot Western blotting. Results The specific activity of caspase-3 (1.00 ± 0.04) in adult rats was significantly higher than that in aged rats (2.31 ± 0.43, P <0.01). The expression of Omi / HtrA2 in myocardium of aged rats was significantly increased, and the expression of XIAP was significantly decreased. Given a specific Omi / HtrA2 inhibitor ucf-101, can significantly reduce the aging rat myocardial tissue caspase-3 expression. Conclusion The increased expression of Omi / HtrA2 in the myocardium of aged rats may lead to the degradation of XIAP and subsequent activation of caspase-3 and apoptosis.