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目的研究干旱条件下接种菌根真菌的植物与未接菌植物的蛋白质组差异,探索菌根真菌提高植物抗旱能力可能的机制。方法以未接菌植物作对照,采用双向电泳法首次从真菌、植物和环境3因素上研究在干旱逆境中接种丝核菌属的台湾金线莲与未接菌对照的蛋白质组差异。结果差异表达蛋白中涉及光合作用的有8个,其中7个与暗反应中CO2的固定相关,并且植物启动了干旱条件下的C4途径以充分利用CO2;涉及糖、脂代谢的有5个蛋白,蛋白质合成相关蛋白有3个,抗性相关蛋白2个。结论干旱条件下接菌台湾金线莲的光合作用的暗反应尤其是C4途径加强,增加了在气孔关闭情况下固定和利用CO2的能力,糖、脂代谢和蛋白合成及抵抗病虫害的能力均强于未接菌对照;丝核菌属菌株能够帮助台湾金线莲增加抵御干旱的能力。
Aim To study the proteome difference between plants inoculated with mycorrhizal fungi and non-inoculated plants under drought stress and to explore the possible mechanism of mycorrhizal fungi in improving plant drought tolerance. Methods Two-dimensional electrophoresis was used for the first time to study the proteome difference between A. chinensis and non-inoculated control inoculated with Rhizoctonia in drought stress from three factors of fungi, plant and environment. Results Eight of the differentially expressed proteins involved in photosynthesis, seven of which were related to the fixation of CO2 in the dark reaction and the plant initiated the C4 pathway under drought conditions to fully utilize CO2; five proteins involved in sugar and lipid metabolism , 3 protein synthesis related proteins, 2 resistance related proteins. Conclusion Under dark conditions, the photosynthesis of A. glabrathi dark-response, especially the C4 pathway, is enhanced, and the ability to fix and utilize CO2 under stomatal closure is increased. The ability of sugar and lipid metabolism and protein synthesis and resistance to pests and diseases are enhanced In non-inoculated control; Rhizoctonia strains can help Taiwan Anoectochilus increase drought-resistant capacity.