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在急性白血病的细胞遗传学分析中,已常规进行骨髓和外周血培养.但常因白细胞数低而致培养失败,或出现染色体严重收缩,分裂期细胞展开后质量差.本文报告用氯化铵预处理患者的骨髓和血液,常能得到在质量上类似于正常刺激后血培养的染色体标本.氯化铵预处理的改进方法简便,操作时把骨髓和血液标本中加无菌氯化铵后再短期培养即可,至今作者已用该方法分析了100例,其中最初15例血液或骨髓标本分别用氯化铵预处理后培养方法和常规骨髓培养方法重复培养来作为对照.结果:经氯化铵预处理的所有病例标本,细胞分裂指数显著高
In cytogenetic analysis of acute leukemia, bone marrow and peripheral blood cultures have been routinely performed. However, cultures often fail due to low numbers of leukocytes, or severe chromosome chromosomal shrinkage occurs, and the quality of disintegrated cells is poor. This report uses ammonium chloride. Pretreatment of the patient’s bone marrow and blood can often yield chromosomal specimens that are qualitatively similar to blood cultures after normal stimulation. The improved method of ammonium chloride pretreatment is simple, and after adding sterile ammonium chloride to bone marrow and blood specimens during operation, Short-term culture can be done. So far, the authors have used this method to analyze 100 cases, of which the first 15 blood or bone marrow specimens were cultured with ammonium chloride pretreatment and conventional bone marrow culture methods as a control. Results: Chlorine All cases of ammonium pretreatment specimens, the cell division index was significantly higher