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目的 :排除基质的干扰 ,建立一种测定氟尿嘧啶软膏的方法。方法 :应用三波长分光光度法 ,紫外检测波长为 2 93nm ,2 6 5nm和 2 5 6nm。结果 :平均回收率为 99 9% ,RSD为 0 8% (n =5 )。结论 :该法快速准确 ,可作为氟尿嘧啶软膏的含量测定方法
OBJECTIVE: To exclude matrix interference and to establish a method for the determination of fluorouracil ointment. Methods: Three-wavelength spectrophotometry was used to detect UV-vis wavelengths at 293 nm, 265 nm and 256 nm. Results: The average recovery was 99.9% with a RSD of 0.8% (n = 5). Conclusion: The method is rapid and accurate and can be used as a method for the determination of fluorouracil ointment