选用正常足月妊娠新鲜胎盘,经酶消化合并机械分离法分离胎盘合体滋养细胞。所获得的合体滋养细胞的纯度达90～95％以上,细胞活力大于90％。经培养4小时的合体滋养细胞的上清液,对母亲与胎儿的淋巴细胞转化试验和向混合淋巴细胞培养均有明显的抑制。提示可能通过局部抑制母体免疫 Selection of normal full-term pregnancy of the newborn placenta, enzymatic digestion combined with mechanical separation of placental syncytiotrophoblast cells. The resulting syncytiotrophoblast purity of 90 ~ 95%, cell viability greater than 90%. Supernatants of syncytiotrophoblasts cultured for 4 hours showed significant inhibition on the lymphocyte transformation test of mother and fetus and mixed lymphocyte culture. Tip may be through local inhibition of maternal immunity