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以酪蛋白和明胶为蛋白源,豆油为脂肪源配制团头鲂幼鱼基础饲料,制成维生素C水平为0.2、33.4、65.8、133.7、251.5mg/kg和501.5 mg/kg的6种等氮等能的试验饲料。每种试验饲料随机投喂3组实验团头鲂幼鱼,每天投喂3次,达饱食水平,试验周期为90 d。结果表明,随着饲料中维生素C添加量的增加,增重率(WG)、特定生长率(SGR)呈先上升而后趋于平缓的趋势,其中251.5 mg/kg和501.5 mg/kg Vc添加组团头鲂WG、SGR显著高于对照组(P<0.05)。各组间肝体比(HSI)和肥满度(CF)无显著性差异,仅133.7 mg/kg Vc添加组团头鲂脏体比(VSI)显著高于对照组(P<0.05)。与对照组相比,饲料中添加65.8～501.5mg/kg Vc显著降低了血液中白细胞(WBC)数量;添加65.8 mg/kgVc显著降低了血清谷草转氨酶(AST)活力,133.7 mg/kg和501.5mg/kg试验组显著降低了谷丙转氨酶(ALT)的水平;添加501.5mg/kg Vc显著提高了团头鲂血清中甘油三酯(TG)的水平;添加133.7 mg/kg Vc显著提高了肌肉中SOD活性(P<0.05)。饲料中维生素C不同添加量对团头鲂幼鱼成活率、血液中红细胞(RBC)数量、血清碱性磷酸酶(ALP)水平、肌肉蒸煮损失等均无显著影响(P>0.05)。以鱼体增重率为评价指标,经折线模型回归分析,得到团头鲂幼鱼饲料中Vc的适宜添加量为150 mg/kg。
Casein and gelatin were used as the protein source and soybean oil as fat source to prepare the basal diet of juvenile fish. Six kinds of isosilates with the vitamin C levels of 0.2, 33.4, 65.8, 133.7, 251.5 mg / kg and 501.5 mg / kg Can wait for the experimental feed. Each experimental group was fed three groups of experimental pigs randomly fed three times a day, up to full feeding level, the test period was 90 days. The results showed that the weight gain (WG) and the specific growth rate (SGR) increased first and then tended to be gentle with the increase of dietary vitamin C concentration, and 251.5 mg / kg and 501.5 mg / kg Vc Head 鲂 WG, SGR was significantly higher than the control group (P <0.05). There were no significant differences in HSI and CF between the two groups. The VSI of 133.7 mg / kg Vc group was significantly higher than that of the control group (P <0.05). Compared with the control group, adding 65.8-501.5mg / kg Vc to the diet significantly reduced the number of white blood cells (WBC) in the blood; adding 65.8 mg / kg Vc significantly reduced serum AST, 133.7 mg / kg and 501.5 mg / kg significantly reduced the level of alanine aminotransferase (ALT); addition of 501.5mg / kg Vc significantly increased serum triglyceride (TG) levels; add 133.7 mg / kg Vc significantly increased muscle SOD activity (P <0.05). There was no significant difference between the dietary vitamin C supplementation and the survival rate of RBC, RBC, serum ALP and muscle cooking loss (P> 0.05). With the weight gain rate of fish as the evaluation index, the regression equation of the polyline model was used to get the suitable dosage of Vc for 150 mg / kg.