为了探讨Survivin负性突变体T34A对乳腺癌细胞MCF-7增殖与凋亡的影响,本文分别采用生理盐水、空载体质粒(PORF-9-null)及Survivin-T34A与MCF-7细胞共培养,观察体外MCF-7细胞的增殖及凋亡情况;建立乳腺癌裸鼠模型,体内实验检测Survivin-T34A对乳腺癌的抑制作用。结果表明转染了Survivin-T34A的细胞增殖率明显低于PORF-9-null组,电镜下观察可见细胞凋亡增多,流式细胞术检测显示其凋亡率明显高于空载体组。瘤内注射Survivin-T34A组的抑瘤效果明显,抑制率达47.1%。本研究提示Survivin-T34A负性突变体能够有效地抑制乳腺癌细胞的增殖,并促进其凋亡,从而发挥抗肿瘤作用。 To investigate the effect of Survivin negative mutant T34A on the proliferation and apoptosis of breast cancer cell line MCF-7, normal saline, empty vector plasmid (PORF-9-null) and Survivin-T34A were co-cultured with MCF- To observe the proliferation and apoptosis of MCF-7 cells in vitro; To establish a model of breast cancer in nude mice and to test the inhibitory effect of Survivin-T34A on breast cancer in vivo. The results showed that the proliferation rate of Survivin-T34A transfected cells was significantly lower than that of PORF-9-null cells. The apoptosis of cells was observed under electron microscope. The apoptosis rate of Survivin-T34A cells was significantly higher than that of empty vector group by flow cytometry. Intratumoral injection of Survivin-T34A group of tumor inhibition effect was obvious, the inhibition rate of 47.1%. This study suggests that Survivin-T34A negative mutant can effectively inhibit the proliferation of breast cancer cells, and promote apoptosis, and thus play an anti-tumor effect.