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利用Western blot技术，检测长途运输试验猪骨骼肌(背最长肌和臀大肌)中分属于HSP70家族和HSP90家族的4种应激蛋白(HSP70，HSP 72，HSP86和HSP90)的表达。所有运输应激猪和对照猪肌肉组织中均检测到了上述4种HSP。对照猪组织中HSP的表达说明HSP除了在受应激的细胞内行使生理功能外，还具有独立于应激刺激应答以外的其它作用。6 h的长途运输后，HSP的表达量明显不同，HSP70和HSP72在肌肉组织中的表达虽然有一定的增加，但统计学分析差异不显著(P＞0.05)；而HSP86和HSP90在骨骼肌中的表达明显下降，尤以HS P90下降最为显著(P＜0.01)。提示HSP90家族成员与肉品品质相关，可能作为判断应激损伤的指征。“,”To demonstrate the expression of heat shock protein s(HSP)in skeletal muscles and to estimate the relationship between expression of HSP and pork quality, using Western blot four kinds of HSP which belongs to HSP70 family and HSP90 family respectively in porcine skeletal muscle tissues were determined after a long distance transport.All four HSP,namely HSP70，HSP72，HSP86 and HSP90，were regularly detected in M.longissimus dorsi and M.gluteus medius of both the transported and the cont rol group.The expression of HSP in control pigs showed that HSPs have some indep endent physiological from stress response undetermined functions.after 6 h trans port,the induction of the HSP70 and HSP72 in skeletal muscle tissues was slightly elevated(P＞0.05).However,expression of the HSP 86 and the HSP90,especially HSP90,markedly decreased(P＜0.01)in skelet al muscles.The results are implied that the reduction tendency of HSP90 fa mily is relative to pork quality and may serve as a potential marker for stress response.