目的和方法：免疫组化和共聚焦激光扫描显微镜观察了不同胚胎发育期的正常和心脏基因突变型美西螈胚胎心脏中波形蛋白的分布和形态。结果：在胚胎第３５期正常心脏中，波形蛋白为粗线条状。第３９期中波形蛋白呈较细的线条状，部分波形蛋白为一个不完全的立体网状结构。到第４１期波形蛋白分布均匀并呈一个立体网状结构。心脏基因突变型胚胎心脏中波形蛋白仅为较细的点状分布，没有分布均匀的网状结构。结论：从结构上来说波形蛋白的分布可能与心肌纤维的发育有关。 PURPOSE AND METHODS: Immunohistochemistry and confocal laser scanning microscopy were used to observe the distribution and morphology of vimentin in normal and cardiac genetically modified embryos of different embryonic stages. RESULTS: In the 35th embryo normal heart, the vimentin was thickly striped. In the 39th period, the vimentin was thinner and the part of the vimentin was an incomplete three-dimensional network. To the first 41 vimentin evenly distributed and showed a three-dimensional network structure. Heart-shaped mutant embryos in the heart of the vimentin only a thin punctate distribution, there is no uniform distribution of the network structure. Conclusion: The structural distribution of vimentin may be related to the development of myocardial fibers.