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本文研究地尔硫 艹卓(Dil)对猪急性心肌梗死 (AMI)面积、降钙素基因相关肽 (CGRP)及内皮素 (ET)的影响。将猪左冠状动脉前降支套扎 6 0min ,恢复血流 180min ,造成急性心肌缺血 /再灌注 (MI/RI)动物模型。随机化分为Dil组 [Dil组 ,Diltiazem 10 0 μg/ (kg·min) ],及对照组 (Con组 ,生理盐水 ) ,均采用冠状静脉逆行灌注法 (CVRI) ,于缺血后5 0min给Dil或生理盐水以 2mL/min速度持续 40min。实验过程中监测心率及平均动脉压变化。测定心肌梗死面积和冠状静脉血浆CGRP及ET含量。结果表明 ,与对照组比较 ,Dil能显著减慢心率 (73 6± 19 8比 110 3± 16 5 ,P <0 0 5 )、减少ET升高浓度 (392 7± 5 0 6比 437 1± 43 1,P <0 0 5 )、降低CGRT下降浓度 (38 2 5± 7 70比 2 9 2 1± 7 76 ,P <0 0 5 )及心肌梗死面积 [(4 0 3± 7 7) %比 (5 9 2± 8 1) % ,P <0 0 5 ]。
This study investigated the effect of Dil on acute myocardial infarction (AMI) area, calcitonin gene-related peptide (CGRP) and endothelin (ET) in pigs. The left anterior descending coronary ligation of pig 60min, blood flow 180min, resulting in acute myocardial ischemia / reperfusion (MI / RI) animal model. Randomly divided into Dil group (Dil group, Diltiazem 10 0 μg / (kg · min)], and the control group (Con group, normal saline) were retrograde coronary perfusion (CVRI) Dil or saline at a rate of 2 mL / min for 40 min. Heart rate and mean arterial pressure were monitored during the experiment. Myocardial infarction area and coronary venous plasma CGRP and ET contents were determined. The results showed that compared with the control group, Dil significantly reduced heart rate (73 6 ± 19 8 vs. 110 3 ± 165, P <0 05) and decreased ET increased concentration (392 7 ± 50 6 vs 437 1 ± (P <0.05) and myocardial infarct size [(43 0 ± 7 7)%, P <0 05) and decreased the concentration of CGRT (38 2 5 ± 7 70 vs 292 1 ± 7 76, P 0 05) (5 9 2 ± 8 1)%, P <0 0 5].