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目的:研究姜黄素对脂多糖引起的小胶质细胞活化状态的影响,并探讨TOLR4受体在其中的作用。方法:采用体外培养N9小鼠小胶质细胞系,脂多糖作为刺激,激活小胶质细胞。采用ELISA法检测小胶质细胞培养基中TNF-α、IL-1β和IL-6的含量;相差显微镜观测细胞形态;免疫细胞化学和western blot观测小胶质细胞TOLR4受体表达情况。结果:与对照组相比,暴露于100ng/ml脂多糖24 h的小胶质细胞促炎症因子TNF-α、IL1β和IL-6释放量明显增加(P<0.05),姜黄素浓度为10μM时,可显著减少小胶质细胞释放TNF-α、IL-1β和IL-6(P<0.05),此外,姜黄素还可改善小胶质细胞形态,并降低暴露于脂多糖中的小胶质细胞TOLR4受体的表达。结论:姜黄素可能通过抑制TOLR4表达,减轻了脂多糖对小胶质细胞的激活。
Objective: To study the effect of curcumin on microglia activation induced by lipopolysaccharide (LPS) and to explore the role of TOLR4 receptor in it. Methods: N9 mouse microglia cell line was cultured in vitro, lipopolysaccharide was stimulated to activate microglial cells. The contents of TNF-α, IL-1β and IL-6 in microglial medium were detected by ELISA. The morphological changes of cells were observed by phase contrast microscopy. The expression of TOLR4 receptor in microglia was detected by immunocytochemistry and western blot. Results: Compared with the control group, the release of TNF-α, IL1β and IL-6 in microglial cells exposed to 100ng / ml lipopolysaccharide increased significantly (P <0.05). When curcumin concentration was 10μM , Significantly decreased the release of TNF-α, IL-1β and IL-6 from microglia (P <0.05). In addition, curcumin also improved microglial morphology and decreased the microglia exposed to lipopolysaccharide Cell TOLR4 receptor expression. Conclusion: Curcumin may reduce the activation of microglia by inhibiting the expression of TOLR4.