【摘 要】
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MicroRNAs (miRNAs) regulate various biological processes during insect development;however,their role in larval-pupal development in oriental fruit fly,Bactroce
【机 构】
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Key laboratory of Horticultural Plant Biology(MOE)and Institute of Urban and Horticultural Entomolog
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MicroRNAs (miRNAs) regulate various biological processes during insect development;however,their role in larval-pupal development in oriental fruit fly,Bactrocera dorsalis (Hendel) remains unknown.In the current study,we address the biological function of a conserved miRNA,Bdo-Let-7 in the regulation of BdE75 gene,which belongs to the ecdysone signaling pathway and participates in the larval-pupal development in B.dorsalis.Using dual luciferase reporter assay in HEK293T cells we show that Bdo-Let-7 miRNA interacts with the 3’untranslated region ofBdE75 gene and suppresses its expression.The Bdo-Let-7 and BdE75 are also co-expressed in the larval-pupal stages and in different tissues ofB.dorsalis.In in vivo experiments,the injection ofBdo-Let-7 agomir and antagomir in third instar larvae down-and up-regulated the expression of BdE75,respectively.The 20-hydroxyecdysone (20E) injection assay shows that 20E up-regulated the expression of Bdo-Let-7 on the 5th day of the larvae.Moreover,abnormal pupation and eclosion were observed after larval Bdo-Let-7 antagomir injection.Based on these results,we show that Bdo-Let-7 regulates the ecdysone signaling pathway through the exact dose of BdE75 gene,and is indispensable for normal larval-pupal development in B.dorsalis.
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