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由于不同肿瘤病人对化疗药物敏感性不同,即使在同一组织类型的肿瘤病人中,个体间对同一药物的敏感性也有差异。因此,用人体肿瘤组织原块培养方法进行抗癌药物敏感试验,可对新药临床试用及医生对某肿瘤病人选择用药提供线索。实验方法如下:将新鲜的肿瘤手术或活检标本,剪成<0.5mm~3小块,放在培养瓶内含有胶原面的盖玻片上,再加入含有20%小牛血清的 Eagle 培养液,置37℃温箱内培养。当显微镜下观察到细胞自组织块向外生长并形成生长晕时,即换以新鲜培养液,同时加入各种受试药物,按
Because different tumor patients have different sensitivity to chemotherapeutic drugs, there is difference in the sensitivity of the same drug among individuals in the same tissue type of tumor patients. Therefore, the use of human tumor tissue culture of the original method of anti-cancer drug sensitivity test, clinical trials of new drugs and doctors can choose to provide clues to a tumor patient. The experimental methods are as follows: fresh tumor surgery or biopsy specimens, cut into small pieces of <0.5mm ~ 3, placed in a flask containing collagen surface cover glass slide, and then added with 20% fetal bovine serum Eagle culture medium 37 ℃ incubator. When observed under the microscope cell growth out of tissue blocks and the formation of growth halo, that is, with fresh culture medium, while adding a variety of drugs under test, press