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目的肺癌 A549和 A549DDP(耐顺铂的 A549)细胞株为体外模型,研究 As_2O_3对 A549和 A549DDP 细胞的生物学效应机制。方法四甲基偶氮唑蓝(MTT)比色法检测 A549DDP 细胞对 As_2O_3敏感性;流式细胞仪鉴定凋亡细胞并测定凋亡比率。结果 As_2O_3明显抑制 A549细胞的
Objective To investigate the biological effect of As_2O_3 on A549 and A549DDP cells in vitro and in vitro. Methods MTT assay was used to detect the sensitivity of A549DDP cells to As 2 O 3. Apoptotic cells were identified by flow cytometry and the apoptosis rate was determined. Results As 2 O 3 significantly inhibited A549 cells