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神经氨酸酶(NA)是一种具有唾液酸酶活性的流感毒粒表面糖蛋白,切断病毒HA与细胞膜上神经氨酸残基之间的连接,使病毒能够从宿主细胞表面释放.检测了新型H1N1流感NA基因核苷酸序列,用免疫信息学方法预测、筛选和鉴定B细胞表位;人工合成NA抗原多肽SE8和RE6,并免疫新西兰兔制备抗血清.两种抗血清具有体外中和新型H1N1流感病毒能力(微量中和实验),而且还具有拮抗血凝释放作用.根据2009年全球毒株NA基因序列检测和比对结果,发现多肽SE8和RE6序列未变异.实验揭示,多肽SE8和RE6可以作为新型H1N1流感候选多肽疫苗.
Neuraminidase (NA), a secreted virion surface glycoprotein with sialidase activity, cleaves the link between the viral HA and the neuraminic acid residues on the cell membrane, allowing the virus to be released from the host cell surface. The nucleotide sequence of NA gene of novel H1N1 influenza was used to predict, screen and identify B cell epitopes by immunological informatics method. Synthesized NA antigen peptides SE8 and RE6 and immunized New Zealand rabbits to prepare antiserum.The two antisera had the function of in vitro neutralization New H1N1 influenza virus (micro-neutralization test), but also has the role of antagonizing the release of blood clotting.According to the detection and comparison of the NA gene sequence of the global strain in 2009, it was found that the sequence of the polypeptide SE8 and RE6 did not change.The experiment revealed that the polypeptide SE8 And RE6 can be used as novel H1N1 influenza candidate polypeptide vaccine.