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植物LEC蛋白是NF-Y转录因子的一类B亚基,在植物胚状体形成过程中起重要作用。为了研究大麦小孢子体外培养形成胚状体的机理,本研究利用RACE技术在大麦中克隆了一个新的LEC基因,该基因cDNA全长为1004 bp,开放阅读框全长为597 bp,编码198个氨基酸,其蛋白1~59位氨基酸含有LEC结构域,命名为HvLEC1。HvLEC1在大麦的根、茎、叶和小孢子培养过程中均能表达,其中小孢子培养7 d时表达量最高,且HvLEC1在大麦品系BI04中的表达量比基19高,BI04愈伤产量也比基19高,表明HvLEC1表达量和愈伤产量有相关性,受盐胁迫后HvLEC1在大麦的根中快速上调表达,提示HvLEC1可能不仅参与小孢子胚状体发生,而且参与盐胁迫响应。
Plant LEC protein is a type B subunit of NF-Y transcription factor that plays an important role in plant embryoid body formation. In order to study the mechanism of embryoid body formation of microspore in vitro, a new LEC gene was cloned in barley by RACE technique. The full-length cDNA was 1004 bp in length and the full-length open reading frame was 597 bp encoding 198 Amino acids, the protein 1-59 amino acids contain LEC domain, named HvLEC1. HvLEC1 was expressed in the roots, stems, leaves and microspore cultures of barley, of which microspores had the highest expression level on the 7th day and the expression level of HvLEC1 in the barley line BI04 was higher than that of the base 19. The callus yield of BI04 Higher than the base 19, indicating that there is a correlation between the expression level of HvLEC1 and the callus yield. HvLEC1 rapidly up-regulated in the root of barley after salt stress, suggesting that HvLEC1 may participate not only in microspore embryogenesis but also in response to salt stress.