论文部分内容阅读
目的:观察阿司匹林对体外培养的正常人表皮黑素细胞系PI G1细胞的活力、黑素生成及酪氨酸酶活性的影响。方法:用不同浓度的阿司匹林处理PIG1细胞72h,CCK-8比色法测定阿司匹林对PIG1细胞活力的影响;光学显微镜观察细胞形态学变化;氢氧化钠裂解法测定黑素的含量;体外多巴氧化反应法测定酪氨酸酶活性的变化。结果:阿司匹林浓度小于或者等于500μmol/L时无明显细胞毒性;浓度大于或者等于125μmol/L时以剂量依赖方式抑制黑素生成,差异有统计学意义(P<0.05);黑素细胞酪氨酸酶活性无显著性变化(P>0.05)。结论:阿司匹林抑制PIG1细胞的黑素生成,可能应用于色素增多性皮肤病。
Objective: To observe the effect of aspirin on the activity, melanogenesis and tyrosinase activity of normal human epidermal melanocyte line PI G1 cultured in vitro. Methods: PIG1 cells were treated with different concentrations of aspirin for 72 hours. The effect of aspirin on the viability of PIG1 cells was determined by CCK-8 colorimetric assay. Morphological changes of cells were observed by light microscopy. The content of melanin was determined by sodium hydroxide cleavage. Determination of tyrosinase activity by reaction method. Results: Aspirin had no cytotoxicity when its concentration was less than or equal to 500μmol / L; melanin was inhibited in a dose-dependent manner when its concentration was greater than or equal to 125μmol / L, the difference was statistically significant (P <0.05); tyrosine No significant changes in enzyme activity (P> 0.05). Conclusion: Aspirin inhibits the melanogenesis of PIG1 cells and may be used in pigmented skin diseases.